Anti-SEMA4B抗体(ab81130)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SEMA4B
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 182-231 (KGRCPFDPNF KSTALVVDGE LYTGTVSSFQ GNDPAISRSQ SLRPTKTESS) of human SEMA4B (NP_064595).

  • 阳性对照
    • Fetal membrane lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    SEMA4B belongs to the semaphorin family and contains 1 Ig-like C2-type (immunoglobulin-like) domain, 1 PSI domain and 1 Sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • 细胞定位
    Membrane
  • 数据库链接
  • 别名
    • KIAA1745 antibody
    • MGC131831 antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B antibody
    • SEMA4B antibody
    • SEMAC antibody
    • Semaphorin 4B antibody
    • SemC antibody
    see all

图片

  • Anti-SEMA4B antibody (ab81130) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Anti-SEMA4B antibody (ab81130) at 1.6 µg/ml + MIA-PACA2 cells

    Predicted band size : 93 kDa

    WB Suggested Anti-WBP11 antibody (ab85563) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain.

    Secondary antibody - anti-rabbit HRP (ab6721)

实验方案

文献

ab81130 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1% NaAz in PBS
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Verified customer

提交于 Apr 30 2014

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Abcam user community

Verified customer

提交于 Jan 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HEK293 cells transfected with mouse Sema4B express)
Specification
HEK293 cells transfected with mouse Sema4B express
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

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Verified customer

提交于 Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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