Anti-SEMA4A抗体(ab70178)
Key features and details
- Rabbit polyclonal to SEMA4A
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-SEMA4A抗体
参阅全部 SEMA4A 一抗 -
描述
兔多克隆抗体to SEMA4A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Mouse, Rat -
免疫原
A synthesized peptide derived from the internal region of human SEMA4A.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70178于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/50 - 1/100. |
靶标
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功能
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. -
疾病相关
Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) [MIM:610282]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in SEMA4A are the cause of cone-rod dystrophy type 10 (CORD10) [MIM:610283]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. -
序列相似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 64218 Human
- Entrez Gene: 20351 Mouse
- Entrez Gene: 310630 Rat
- Omim: 607292 Human
- SwissProt: Q9H3S1 Human
- SwissProt: Q62178 Mouse
- Unigene: 408846 Human
- Unigene: 439752 Mouse
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别名
- CORD10 antibody
- RP11 54H19 2 antibody
- RP35 antibody
see all
图片
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All lanes : Anti-SEMA4A antibody (ab70178) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from COS-7 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 117 kDa, 26 kDa. We are unsure as to the identity of these extra bands.
One other band detected greater then 117 kDa. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab70178 at 1/50-1/100 dilution.
Left image un-treated.Right image treated with immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70178 被引用在 3 文献中.
- Sukumaran SK et al. Whole transcriptome profiling of taste bud cells. Sci Rep 7:7595 (2017). PubMed: 28790351
- Wang L et al. Expression of Semaphorin 4A and its potential role in rheumatoid arthritis. Arthritis Res Ther 17:227 (2015). ELISA ; Human . PubMed: 26303122
- Vadasz Z et al. The involvement of immune semaphorins and neuropilin-1 in lupus nephritis. Lupus 20:1466-73 (2011). IHC-P ; Human . PubMed: 21951945