antigen sequence: CMMVFFLSNM IENQCMSTGA FEITLNDVPV WSKLESGHLP SMQQLVQILD NEMKLNVHMD SIPHHR, corresponding to C terminal amino acids 129-194 of Human SELT.
- Human hippocampus tissue.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the SelWTH family. SELT subfamily.
May contain a selenide-sulfide bond between Cys-46 and Sec-49. This bond is speculated to serve as redox active pair.
Information by UniProt
- Selenoprotein T antibody
- SelT antibody
- SELT_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-SELT antibody (ab122587)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122587 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab122587)
ab122587, at 1/10 dilution, staining SELT in paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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