概述

  • 产品名称Anti-Seladin 1抗体
    参阅全部 Seladin 1 一抗
  • 描述
    兔多克隆抗体to Seladin 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Seladin 1.

    (Peptide available as ab41486.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue Lysates: Brain, Heart and Skeletal Muscle.

应用

Our Abpromise guarantee covers the use of ab40490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能Catalyzes the reduction of the delta-24 double bond of sterol intermediates. Protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.
  • 组织特异性Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.
  • 通路Steroid biosynthesis; cholesterol biosynthesis.
  • 疾病相关Desmosterolosis (DESMOS) [MIM:602398]: Rare autosomal recessive disorder characterized by multiple congenital anomalies and elevated levels of the cholesterol precursor desmosterol in plasma, tissue, and cultured cells. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 序列相似性Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
    Contains 1 FAD-binding PCMH-type domain.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 24 dehydrocholesterol reductase antibody
    • 24-dehydrocholesterol reductase antibody
    • 3 beta hydroxysterol delta 24 reductase antibody
    • 3-beta-hydroxysterol delta-24-reductase antibody
    • DCE antibody
    • Delta(24)-sterol reductase antibody
    • Desmosterol to cholesterol enzyme antibody
    • DHC24_HUMAN antibody
    • Dhcr24 antibody
    • Diminuto dwarf1 homolog antibody
    • Diminuto/dwarf1 homolog antibody
    • KIAA0018 antibody
    • Nbla03646 antibody
    • Seladin-1 antibody
    • SELADIN1 antibody
    • Selective AD indicator 1 antibody
    • Selective Alzheimer's Disease Indicator 1 antibody
    see all

Anti-Seladin 1 antibody 图像

  • All lanes : Anti-Seladin 1 antibody (ab40490) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab40490 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40490, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-Seladin 1 antibody (ab40490)参考文献

This product has been referenced in:
  • Ishida E  et al. Crosstalk between thyroid hormone receptor and liver X receptor in the regulation of selective Alzheimer's disease indicator-1 gene expression. PLoS One 8:e54901 (2013). WB ; Mouse . Read more (PubMed: 23359226) »
  • Ishida E  et al. Thyroid hormone receptor and liver X receptor competitively up-regulate human selective Alzheimer's disease indicator-1 gene expression at the transcriptional levels. Biochem Biophys Res Commun 432:513-8 (2013). Read more (PubMed: 23416078) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (adrenal)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate ph6.0, 20 min 100C
Permeabilization No
Specification adrenal
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 02 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart, Intestine, Kidney)
Loading amount 25 µg
Specification Heart, Intestine, Kidney
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"