概述

  • 产品名称Anti-SEL1L抗体
    参阅全部 SEL1L 一抗
  • 描述
    兔多克隆抗体to SEL1L
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SEL1L.

    (Peptide available as ab86659.)

  • 阳性对照
    • This antibody gave a positive signal in HepG2 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab78298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/2000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).

SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).

靶标

Anti-SEL1L antibody 图像

  • Anti-SEL1L antibody (ab78298) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78298 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).

  • ab78298 staining SEL1L in Human pancreatic tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 5 minutes of peroxidase followed by 10 minutes of protein at 20°C; antigen retrieval was by heat mediation in target retrieval solution. The sample was incubated with primary antibody (1/2000 in antibody diluent) at 20°C for 45 minutes. An undiluted HRP-conjugated polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

  • All lanes : Anti-SEL1L antibody (ab78298) at 1 µg/ml

    Lane 1 : Mouse Pancreas
    Lane 2 : Mouse Liver
    Lane 3 : Mouse Heart

    Lysates/proteins at 20 µg per lane.

    Secondary
    Swine anti-Rabbit HRP at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24& 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is courtesy of an abreview submitted by Antibody Solutions Ltd.

    See Abreview

Anti-SEL1L antibody (ab78298)参考文献

This product has been referenced in:
  • Ji Y  et al. The Sel1L-Hrd1 Endoplasmic Reticulum-Associated Degradation Complex Manages a Key Checkpoint in B Cell Development. Cell Rep 16:2630-40 (2016). WB . Read more (PubMed: 27568564) »
  • Sun S  et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A 111:E582-91 (2014). Read more (PubMed: 24453213) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (3T3)
Specification 3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Dr. Marina Shenkman

Verified customer

提交于 Apr 27 2012

Thank you for your reply, and I apologize for the delay as I was out of the office yesterday.
I have issued the code: ***. This code will become active as soon as the review for ab78298 in ICC/IF is submitted, and it can be redeemed for a free pri...

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Thank you for your reply, and I'm sorry to hear that the suggestions did not help improve the results.
We have not tested ab78298 in ICC/IF, and so we can not guarantee the results of the antibody in this application. Since the antibody worked in ...

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Thank you for contacting us and for letting us know about the customer's trouble with this antibody.
It is possible that the fixation and permeabilization methods will need to be optimized, as we have not tested the antibody in IF in our own lab. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Ms Pancreas Ms Liver Ms Heart)
Loading amount 20 µg
Specification Ms Pancreas Ms Liver Ms Heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Pancreas)
Specification Human Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Mar 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"