概述

  • 产品名称Anti-Securin抗体
    参阅全部 Securin 一抗
  • 描述
    兔多克隆抗体to Securin
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Horse, Cat, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (

    ATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFD

    ) of Human Securin (NP_004210).

  • 阳性对照
    • COLO205 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab94689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • 组织特异性Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • 序列相似性Belongs to the securin family.
  • 发展阶段Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • 结构域The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • 翻译后修饰Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW555095 antibody
    • C87862 antibody
    • Cut2 antibody
    • EAP 1 antibody
    • EAP1 antibody
    • ESP1 associated protein 1 antibody
    • Esp1-associated protein antibody
    • hPTTG antibody
    • MGC126883 antibody
    • MGC138276 antibody
    • Pds1 antibody
    • Pituitary tumor transforming 1 antibody
    • Pituitary tumor transforming protein 1 antibody
    • Pituitary tumor-transforming 1, isoform CRA_a antibody
    • Pituitary tumor-transforming 1, isoform CRA_b antibody
    • Pituitary tumor-transforming gene 1 antibody
    • Pituitary tumor-transforming gene 1 protein antibody
    • PTTG 1 antibody
    • PTTG antibody
    • PTTG1 antibody
    • PTTG1 protein antibody
    • PTTG1_HUMAN antibody
    • Pttg3 antibody
    • Securin antibody
    • Tumor transforming 1 antibody
    • Tumor transforming protein 1 antibody
    • Tumor-transforming protein 1 antibody
    • TUTR 1 antibody
    • TUTR1 antibody
    see all

Anti-Securin antibody 图像

  • Anti-Securin antibody (ab94689) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Gel concentration: 12%
  • Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Securin with ab94689 5µg/ml.

Anti-Securin antibody (ab94689)参考文献

ab94689 has not yet been referenced specifically in any publications.

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