The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Dilute 10µl of reconstituted antibody to 10ml(1/1000) with any buffer suitable for your experiment. Detects a band of approximately 15 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
组织特异性Heart, placenta and less abundantly, in lung.
序列相似性Belongs to the phospholipase A2 family.
翻译后修饰This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] 图像
Western blot - Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708)
All lanes : Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708) at 1/1000 dilution
Lane 2 : sPLA2 (Type II) at 1 µg Lane 3 : A10 smooth muscle lysate (iPLA2) at 20 µg Lane 4 : Bee venom sPLA2 at 1 µg Lane 5 : Semi-pure cPLA2 at 5 µg Lane 6 : sPLA2 (Type V) at 0.04 µg Lane 7 : sPLA2 (Type V) at 0.008 µg