Anti-Secretory phospholipase A2 Type V抗体(ab23709)


  • 产品名称Anti-Secretory phospholipase A2 Type V抗体
    参阅全部 Secretory phospholipase A2 Type V 一抗
  • 描述
    兔多克隆抗体to Secretory phospholipase A2 Type V
  • 特异性(+) Human, murine, and rat sPLA2 (Type V); (-) bee venom and human synovial sPLA2 (Type II), iPLA2 (A10 cell lysate), and cPLA2 (HeLa cell lysate); sPLA2 (Type X) not tested, but is expected to be negative (peptide antigen is 12.5% homologous to murine and rat sPLA2 (Type X) and 25% homologous to human (Type II) and (Type X) sPLA2).
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 79-94 of Mouse Secretory phospholipase A2 Type V.

  • 阳性对照
    • Expressed in heart, lung, placenta and spleen. IHC-P: FFPE human heart tissue sections.


Our Abpromise guarantee covers the use of ab23709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: 1/500. Predicted molecular weight: 15 kDa (Human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
    • 组织特异性Heart, placenta and less abundantly, in lung.
    • 序列相似性Belongs to the phospholipase A2 family.
    • 翻译后修饰This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ca2+ dependent phospholipase A2 antibody
      • Calcium dependent phospholipase A2 antibody
      • Calcium-dependent phospholipase A2 antibody
      • DKFZp686C2294 antibody
      • FRFB antibody
      • Group V phospholipase A2 antibody
      • GV PLA2 antibody
      • gVPLA2 antibody
      • hVPLA(2) antibody
      • MGC46205 antibody
      • OTTHUMP00000044655 antibody
      • PA2G5_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase antibody
      • Phosphatidylcholine 2-acylhydrolase 5 antibody
      • Phospholipase A2 group V antibody
      • PLA2 10 antibody
      • PLA2 G5 antibody
      • PLA2-10 antibody
      • Pla2g5 antibody
      • sPLA2 Type V antibody
      see all

    Anti-Secretory phospholipase A2 Type V antibody 图像

    • IHC image of Secretory phospholipase A2 Type V staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23709, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Secretory phospholipase A2 Type V antibody (ab23709) at 1/500 dilution

      Lane 1 : Recombinant human sPLA2 (type V) (100 ng)
      Lane 2 : Recombinant human sPLA2 (type V) (50 ng)
      Lane 3 : Mouse heart homogenate (50ug)

      Predicted band size : 15 kDa
      Observed band size : 15 kDa

    Anti-Secretory phospholipase A2 Type V antibody (ab23709)参考文献

    ab23709 has not yet been referenced specifically in any publications.

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