Phospholipase A2 Assay试剂盒(ab133089)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Plasma
概述
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产品名称
Phospholipase A2 Assay试剂盒
参阅全部 Phospholipase A2 试剂盒 -
检测方法
Colorimetric -
样品类型
Plasma -
检测类型
Enzyme activity (quantitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Phospholipase A2 Assay Kit (ab133089) provides an accurate and convenient method for measurement of PLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5’-dithio-bis-(2-nitrobenzoic acid)).
If you want to detect cytosolic PLA2, we recommend using Cytosolic Phospholipase A2 Assay Kit (ab133090).
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 96 tests 96-Well Plate (Colorimetric Assay) 1 x 96 tests 96-Well Plate Cover 1 unit Bee venom PLA2 Control 1 vial PLA2 Assay Buffer (10X) 1 vial PLA2 Diheptanoyl Thio-PC (Substrate) 2 vials PLA2 DTNB 4 vials -
研究领域
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功能
Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
序列相似性
Belongs to the phospholipase A2 family. -
细胞定位
Membrane. - Information by UniProt
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别名
- EC 3.1.1.4
- GIIC sPLA2
- Group IIA phospholipase A2
see all
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133089 被引用在 3 文献中.
- Koundouros N et al. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell 181:1596-1611.e27 (2020). PubMed: 32559461
- Tamayose CI et al. Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema. Int J Mol Sci 18:N/A (2017). PubMed: 28906474
- Lewin M et al. Varespladib (LY315920) Appears to Be a Potent, Broad-Spectrum, Inhibitor of Snake Venom Phospholipase A2 and a Possible Pre-Referral Treatment for Envenomation. Toxins (Basel) 8:N/A (2016). PubMed: 27571102