Anti-Secretory Component Glycoprotein抗体[SC-05] (ab3924)

概述

  • 产品名称Anti-Secretory Component Glycoprotein抗体[SC-05]
    参阅全部 Secretory Component Glycoprotein 一抗
  • 描述
    小鼠单克隆抗体[SC-05] to Secretory Component Glycoprotein
  • 特异性MAb SC-05 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA).
  • 经测试应用适用于: IHC-Fr, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (affinity-purified secretory component) (Human colostrum).

  • 常规说明


    Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • Primary antibody说明Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
  • 克隆单克隆
  • 克隆编号SC-05
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). See Abreview. Use under non-reducing conditions.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
  • 组织特异性Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.
  • 疾病相关Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ECM 1 antibody
    • Ecm1 antibody
    • ECM1_HUMAN antibody
    • Extracellular matrix protein 1 antibody
    • Secretory component p85 antibody
    • URBWD antibody
    see all

Anti-Secretory Component Glycoprotein antibody [SC-05] 图像

  • All lanes : Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab3924 staining Secretory Component Glycoprotein in human normal breast tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, blocked using 10% serum for 5 minutes at 25°C and then incubated with ab3924 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    The images show stainings of normal breast glands from two different patients. The antibody mostly stains the apical/luminal region of luminal epithelial cells, but the intensity varies from patient to patient.

    See Abreview

  • Immunohistochemical analysis of Human breast cancer tissue, staining Secretory Component Glycoprotein with ab3924.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Tissue was blocked with 10% normal goat serum before incubating with primary antibody (1/50) for 2 hours at room temperature. Staining was primarily in the cytoplasm.

Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924)参考文献

This product has been referenced in:
  • Wu Q  et al. Extracellular matrix protein 1 is correlated to carcinogenesis and lymphatic metastasis of human gastric cancer. World J Surg Oncol 12:132 (2014). IHC-P ; Human . Read more (PubMed: 24779890) »
  • Wu QW  et al. Expression and clinical significance of extracellular matrix protein 1 and vascular endothelial growth factor-C in lymphatic metastasis of human breast cancer. BMC Cancer 12:47 (2012). IHC-P ; Human . Read more (PubMed: 22284579) »

See all 6 Publications for this product

Product Wall

I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

The difference ...

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It appears that there is a nomenclature difference between your target of interest (secretory component) and our antibodies that are anti "secretory component glycoprotein" which is also referred to as "Extracellular matrix protein 1" and "secretory co...

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For ab3924, the links to the databases and target information mention "Extracellular matrix protein 1" which is an alternative name for "secretory component p85" and "secretory component glycoprotein" according to the uniprot file below:
http://www...

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Thank you for contacting us.

In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), so...

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Thank you for taking the time to respond and for contacting Abcam.

With over 70,000 products it is logistically impossible for us to offer free samples. I hope you understand.

Please let me know if you have any questions.

Thank you for contacting Abcam.

I am sorry to hear that Ab55676 has not performed well for you. Although the peptide sequence has excellent identity with the mouse homolog, this product is not guaranteed to work in mouse tissues. High backgro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Normal breast)
Specification Normal breast
Fixative Methanol
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jan 04 2012

Thank you for contacting us. Unfortunately we do not have any specific information about the epitope of Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924). We do however know that the epitope recognised is reduction-resistant and prese...

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Thank you for your enquiry. Unfortunately the only information the originator of the antibody was able to provide was mostly on the datasheet: The antibody reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory ...

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Thank you for your enquiry. There is no information currently available about the working dilution for this antibody in IHC and Western blot. For a purified antibody, we recommend starting dilutions for Western blot to be 1 ug/mL and for IHC, 10 ug/mL....

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