Anti-Secretogranin V抗体(ab55687)


  • 产品名称Anti-Secretogranin V抗体
    参阅全部 Secretogranin V 一抗
  • 描述
    小鼠单克隆抗体to Secretogranin V
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 59-160 of Human Secretogranin V with GST tag



Our Abpromise guarantee covers the use of ab55687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Known variously as APPG, 7B2 and neuroendocrine protein I, Secretogranin V was originally isolated from porcine pituitary in 1983. It soon became evident that Secretogranin V-like immunoreactants were present in many cell types from a variety of species, and it is now recognised to be widely distributed in neural and endocrine tissues. In the early 1990s full length (27kDa) Secretogranin V was shown to be a potent inhibitor of prohormone convertase PC2 activity, a subtilisin-like enzyme responsible for the proteolytic cleavage of many neuroendocrine precursor molecules, including proenkephalin, POMC and gastrin-17. mRNA distribution analysis revealed that in adult rat brain, expression of Secretogranin V mRNA was pan-neuronal, whereas PC2 mRNA was exclusively neuronal, but more restricted. PC2+/Secretogranin V- cells were not encountered in the adult, but were common in developing rat brain. These findings suggest that Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a C-terminal peptide. It is the C-terminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable.
    • 细胞定位Neuroendocrine and endocrine secretory granules.
    • 数据库链接
    • 别名
      • 7B2 antibody
      • 7B2 protein antibody
      • APPG antibody
      • Neuroendocrine protein 7B2 antibody
      • P7B2 antibody
      • Pituitary polypeptide antibody
      • Prohormone convertase chaperone antibody
      • SCG 5 antibody
      • SCG5 antibody
      • Secretogranin 5 antibody
      • Secretogranin V 7B2 protein antibody
      • Secretogranin5 antibody
      • SecretograninV antibody
      • Secretory granule endocrine protein I antibody
      • Secretory granule neuroendocrine protein 1 7B2 protein antibody
      • Secretory granule neuroendocrine protein 1 antibody
      • Sg V antibody
      • SGNE 1 antibody
      • SGNE1 antibody
      • SgV antibody
      see all

    Anti-Secretogranin V antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab55687 Secretogranin V antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa (MW of the GST tag alone is 26kDa)

    Anti-Secretogranin V antibody (ab55687)参考文献

    ab55687 has not yet been referenced specifically in any publications.

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