Anti-SECISBP2抗体(ab53534)

概述

  • 产品名称Anti-SECISBP2抗体
    参阅全部 SECISBP2 一抗
  • 描述
    山羊多克隆抗体to SECISBP2
  • 经测试应用适用于: WB, ICC/IF, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-KERQERKQRLQEN

    , corresponding to internal sequence amino acids 538-550 of Human SECISBP2

应用

Our Abpromise guarantee covers the use of ab53534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml.

靶标

  • 功能Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB.
  • 组织特异性Expressed at high levels in testis.
  • 疾病相关Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) [MIM:609698]. This phenotype is associated with a reduction in type II iodothyronine deiodinase activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686C09169 antibody
    • OTTHUMP00000064929 antibody
    • OTTHUMP00000064930 antibody
    • OTTHUMP00000064931 antibody
    • OTTHUMP00000064932 antibody
    • RP11 89K14.1 antibody
    • SBP 2 antibody
    • SBP2 antibody
    • SEBP2_HUMAN antibody
    • SECIS binding protein 2 antibody
    • SECIS-binding protein 2 antibody
    • SECISBP 2 antibody
    • SECISBP2 antibody
    • Selenocysteine insertion sequence binding protein 2 antibody
    • Selenocysteine insertion sequence-binding protein 2 antibody
    see all

Anti-SECISBP2 antibody 图像

  • All lanes : Anti-SECISBP2 antibody (ab53534) at 1/5000 dilution

    Lane 1 : Mouse testis lysate - cytosolic fraction (50µg)
    Lane 2 : Mouse testis lysate - cytosolic fraction (50µg)
    Lane 3 : Mouse liver lysate - cytosolic fraction (50µg)
    Lane 4 : Mouse kidney lysate - cytosolic fraction (50µg)
    Lane 5 : Mouse brain lysate - cytosolic fraction (50µg)
    Lane 6 : Mouse brain lysate - cytosolic fraction (50µg)
    Lane 7 : Human HepG2 Lysate - cytosolic fraction (50µg)
    Lane 8 : Rat testis lysate - cytosolic fraction (50µg)

    Secondary
    Donkey anti-goat conjugated to HRP

    Predicted band size : 95 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab53534 at 3ug/ml staining SECISBP2 in human testis tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). The tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

Anti-SECISBP2 antibody (ab53534)参考文献

ab53534 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (cytosolic fraction diff. tissues: mouse, rat, huma)
Loading amount 50 µg
Specification cytosolic fraction diff. tissues: mouse, rat, huma
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"