Synthetic peptide, corresponding to a region within amino acids 463-476 of Human SEC61A (NP_037468).
A431, HeLa and HepG2 cell lysates for WB. A431 cells for IF/ICC; Cal27 xenograft for IHC-P
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1X PBS, pH 7
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
1/100 - 1/500.
1/100 - 1/200.
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
Belongs to the secY/SEC61-alpha family.
Endoplasmic reticulum membrane.
Information by UniProt
Western blot - Anti-SEC61A antibody (ab97537)
Anti-SEC61A antibody (ab97537) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 52 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-SEC61A antibody (ab97537)
Immunofluorescence analysis of methanol-fixed A431 cells, using ab97537 at 1/200 dilution (1) and co-stained with Hoescht 33342 (2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61A antibody (ab97537)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab97537 at 1/500 dilution.
has not yet been referenced specifically in any publications.
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