Customer reviews and FAQs

概述

  • 产品名称Anti-SEC61A抗体
    参阅全部 SEC61A 一抗
  • 描述
    兔多克隆抗体to SEC61A
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Pig
    预测可用于: Cow
  • 免疫原

    Synthetic peptide corresponding to Dog SEC61A aa 13-31.
    Sequence:

    CVILPEIQKPERKIQFKEK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Pig microsomes

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 52 kDa).

Detects a band of approximately 38 kDa representing sec61 alpha from pig microsomes.
ICC/IF 1/1000. PubMed: 18784250

靶标

Anti-SEC61A antibody 图像

  • Western blot - SEC61A antibody (ab15575)
    Western blot - SEC61A antibody (ab15575)
    Anti-SEC61A antibody (ab15575) at 1/10000 dilution + Lysates prepared from pig microsomes

    Predicted band size : 52 kDa

Anti-SEC61A antibody (ab15575)参考文献

This product has been referenced in:
  • Buck TM  et al. The Lhs1/GRP170 chaperones facilitate the endoplasmic reticulum-associated degradation of the epithelial sodium channel. J Biol Chem 288:18366-80 (2013). WB . Read more (PubMed: 23645669) »
  • Wang H  et al. Enhanced endoplasmic reticulum entry of tumor antigen is crucial for cross-presentation induced by dendritic cell-targeted vaccination. J Immunol 191:6010-21 (2013). Read more (PubMed: 24218449) »

See all 3 Publications for this product

发表研究结果有使用 ab15575？请让我们知道，以便我们可以引用本数据表中的参考文章。

Product Wall

提交 ab15575 的 abreview 提交 ab15575 的问题

5 Abreviews or Q&A

10
Votes: Highest First

Would like to test this antibody with drosophila samples in IF staining.

DISCOUNT CODE: ***
Expiration date: February 16, 2013
Value: $337

This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab15575 with drosophila samples and i...

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I will attempt to answer your questions below:
1. What's the species you were using to detect the proteins: human,
mouse, etc. proteins with a tag? HEK-293 lysates (human clones with
N-terminal V5-tag). Some abs from abcam (ab60332, ab80...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please find the WB questionnaire below:
1) Abcam product code ab: (ab15575, ab53532, ab96615 and ab106460)

3) Description of the problem: the mentioned antibodies are
non-specific i.e. several bands appear, none of which is the rig...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Product code: 15575
Lot number: GR38115
Inquiry: Dear Sir/Madam, I have previously made orders for primary antibodies (ref: #xxxxx)and #xxxx) and lately realised that they are non-specific. The tests were done by western blot on V5-tagged con...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Dear Tech support,   What are the chances of antibodies ab57523 and ab15575 (or another sec61 antibody) of working with Yeast's proteins WB based on immunogen sequence homology? Thank you for your kind help. Regards,

Thank you very much for your inquiry. Unfortunately we do not have an antibody in our catalogue for the moment for which we can suggest a crossreactivity with the yeast SEC61 proteins. I am very sorry for this. Please see further below for the alignmen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"