概述

  • 产品名称Anti-SEC24D抗体
    参阅全部 SEC24D 一抗
  • 描述
    兔多克隆抗体to SEC24D
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: ASQLILPDSM KVLPVYMNCL LKNCVLLSRP EISTDERAYQ RQLVMTMGVA DSQLFFYPQL LPIHTLDVKS TMLPAAVRCS ESRLS, corresponding to amino acids 830-914 of Human SEC24D.

  • 阳性对照
    • Human pancreas tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab122732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • 组织特异性Ubiquitously expressed, with higher amounts in placenta, pancreas, heart and liver.
  • 序列相似性Belongs to the SEC23/SEC24 family. SEC24 subfamily.
  • 细胞定位Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0755 antibody
    • Protein transport protein Sec24D antibody
    • SC24D_HUMAN antibody
    • SEC24 family member D antibody
    • SEC24 related gene family, member D antibody
    • SEC24-related protein D antibody
    • SEC24D antibody
    see all

Anti-SEC24D antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122732 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122732 at 1/1000 dilution staining SEC24D in Paraffin-embedded Human pancreas by Immunohistochemistry.

Anti-SEC24D antibody (ab122732)参考文献

ab122732 has not yet been referenced specifically in any publications.

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