Anti-Sec24C抗体(ab122635)

概述

  • 产品名称Anti-Sec24C抗体
    参阅全部 Sec24C 一抗
  • 描述
    兔多克隆抗体to Sec24C
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LTKSPVESTT EPPAVRASEE RLSNGDIYLL ENGLNLFLWV GASVQQGVVQ SLFSVSSFSQ ITSGLSVLPV LDNPLSKKVR, corresponding to C terminal amino acids 954-1033 of Human Sec24C.

  • 阳性对照
    • Human pancreas tissue; A431 cells; RT 4, U 251 MG, Human liver and tonsil lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
WB 1/250 - 1/500.

靶标

  • 功能Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the SEC23/SEC24 family. SEC24 subfamily.
  • 细胞定位Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein transport protein Sec24C antibody
    • SC 24C antibody
    • SC24 C antibody
    • SC24C antibody
    • SC24C_HUMAN antibody
    • Sec 24C antibody
    • Sec24 C antibody
    • SEC24 related gene family, member C (S. cerevisiae) antibody
    • SEC24 related protein C antibody
    • SEC24-related protein C antibody
    • SEC24C antibody
    see all

Anti-Sec24C antibody 图像

  • ab122635 at 4 µg/ml staining SEC24C in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
  • ab122635 at 1/10 dilution staining Sec24C in paraffin-embedded human Pancreas tissue by Immunohistochemistry.
  • All lanes : Anti-Sec24C antibody (ab122635) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-Sec24C antibody (ab122635)参考文献

ab122635 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Monkey Cell (Kidney (COS7 cell line))
Specification Kidney (COS7 cell line)
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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提交于 Dec 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"