概述

  • 产品名称Anti-SEC23IP抗体
    参阅全部 SEC23IP 一抗
  • 描述
    兔多克隆抗体to SEC23IP
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residues 350 and 400 of human SEC23IP (NP_009121.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2500 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    Anti-SEC23IP antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SEC23IP with ab70339 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-SEC23IP antibody (ab70339) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 111 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    Anti-SEC23IP antibody (ab70339)参考文献

    ab70339 has not yet been referenced specifically in any publications.

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