Anti-SEC23抗体(ab99552)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SEC23
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Cow, Dog, Human
  • 免疫原

    Synthetic peptide:

    C-ANRAATTGHVID

    , corresponding to internal sequence amino acids 335-346 of Human SEC23

  • 阳性对照
    • NIH 3T3 lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab99552 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).

靶标

  • 功能
    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • 疾病相关
    Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.
  • 序列相似性
    Belongs to the SEC23/SEC24 family. SEC23 subfamily.
  • 细胞定位
    Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLSD antibody
    • Protein transport protein Sec23A antibody
    • SC23A_HUMAN antibody
    • Sec23 homolog A (S. cerevisiae) antibody
    • SEC23-related protein A antibody
    • sec23a antibody
    see all

图片

  • Anti-SEC23 antibody (ab99552) at 2 µg/ml (incubation time 1 hour) + NIH 3T3 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

文献

ab99552 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. Because we carry over 95,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Specification
Panc-1
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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