Customer reviews and FAQs

概述

  • 产品名称
    Anti-SDPR抗体
    参阅全部 SDPR 一抗
  • 描述
    小鼠多克隆抗体to SDPR
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-425 of Human SDPR (NP_004648)

  • 阳性对照
    • transfected 293T cell line

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.

靶标

  • 功能
    May play a role in targeting PRKCA to caveolae.
  • 组织特异性
    Highly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle.
  • 序列相似性
    Belongs to the PTRF/SDPR family.
  • 翻译后修饰
    Phosphorylated on Ser residues.
  • 细胞定位
    Cytoplasm > cytosol. Membrane > caveola. Colocalizes with CAV1 to caveolae.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Cavin 2 antibody
      • Cavin-2 antibody
      • Phosphatidylserine binding protein antibody
      • Phosphatidylserine-binding protein antibody
      • PS p68 antibody
      • PS-p68 antibody
      • Sdpr antibody
      • SDPR_HUMAN antibody
      • SDR antibody
      • Serum deprivation response antibody
      • Serum deprivation response protein antibody
      • Serum deprivation-response protein antibody
      see all

    Anti-SDPR antibody 图像

    • Western blot - SDPR antibody (ab76867)
      Western blot - SDPR antibody (ab76867)
      All lanes : Anti-SDPR antibody (ab76867) at 1/500 dilution

      Lane 1 : SDPR transfected 293T cell line
      Lane 2 : non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 47 kDa
      Observed band size : 72 kDa (why is the actual band size different from the predicted?)

    实验方案

    Anti-SDPR antibody (ab76867)参考文献

    ab76867 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab76867？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab76867 的 abreview 提交 ab76867 的问题

    2 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-SDPR antibody

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (Skeletal muscle cells)
    Specification
    Skeletal muscle cells
    Treatment
    N/A
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Oct 21 2014

    Western blot abreview for Anti-SDPR antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells (ES-E14TG2aATCC))
    Loading amount
    30 µg
    Specification
    Mouse Embryonic Stem Cells (ES-E14TG2aATCC)
    Treatment
    100 µg/ml fibronectin for 24 hrs
    Gel Running Conditions
    Non-reduced Denaturing (gel 10 %)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Aug 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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