概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to SDPR
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-425 of Human SDPR (NP_004648)

  • 阳性对照
    • transfected 293T cell line

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.

靶标

  • 功能
    May play a role in targeting PRKCA to caveolae.
  • 组织特异性
    Highly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle.
  • 序列相似性
    Belongs to the PTRF/SDPR family.
  • 翻译后修饰
    Phosphorylated on Ser residues.
  • 细胞定位
    Cytoplasm > cytosol. Membrane > caveola. Colocalizes with CAV1 to caveolae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cavin 2 antibody
    • Cavin-2 antibody
    • Phosphatidylserine binding protein antibody
    • Phosphatidylserine-binding protein antibody
    • PS p68 antibody
    • PS-p68 antibody
    • Sdpr antibody
    • SDPR_HUMAN antibody
    • SDR antibody
    • Serum deprivation response antibody
    • Serum deprivation response protein antibody
    • Serum deprivation-response protein antibody
    see all

图片

  • All lanes : Anti-SDPR antibody (ab76867) at 1/500 dilution

    Lane 1 : SDPR transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 47 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab76867 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (Skeletal muscle cells)
Specification
Skeletal muscle cells
Treatment
N/A
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 21 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells (ES-E14TG2aATCC))
Loading amount
30 µg
Specification
Mouse Embryonic Stem Cells (ES-E14TG2aATCC)
Treatment
100 µg/ml fibronectin for 24 hrs
Gel Running Conditions
Non-reduced Denaturing (gel 10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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