The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
Use at an assay dependent concentration. PubMed: 20236688
Use a concentration of 5 µg/ml. Detects a band of approximately 28 kDa.
Use a concentration of 1 µg/ml.
功能Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
疾病相关Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors. Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
序列相似性Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.
Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
Succinate dehydrogenase complex subunit B iron sulfur antibody
Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
Succinate dehydrogenase iron sulfur protein antibody
Anti-SDHB antibody [21A11AE7] 图像
Western blot - Anti-SDHB antibody [21A11AE7] (ab14714)
All lanes : Anti-SDHB antibody [21A11AE7] (ab14714)
Lane 1 : Isolated mitochondria from Human heart at 5 µg Lane 2 : Isolated mitochondria from Bovine Heart at 1 µg Lane 3 : Isolated mitochondria from Rat heart at 10 µg Lane 4 : Isolated mitochondria from Mouse heart at 10 µg Lane 5 : Isolated mitochondria from HepG2 cells at 20 µg
Secondary Goat anti-Mouse secondary
Observed band size : 28 kDa (why is the actual band size different from the predicted?) Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [21A11AE7] (ab14714)This image is courtesy of an anonymous Abreview
ab14714 at 5ug/ml staining human adrenal tumor tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. A biotinylated horse anti-mouse IgG antibody was used as the secondary.
ICC/IF image of ab14714 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14714, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Mitochondrial localization of complex II visualized by immunocytochemistry using anti-complex II subunit 30 kDa Ip mAb 21A11 (ab14714). Cells were fixed, permeabilized and then labeled with ab14714 followed by an Alexa Fluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody.
Overlay histogram showing HEK293 cells stained with ab14714 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14714, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-SDHB antibody [21A11AE7] (ab14714)参考文献
This product has been referenced in:
Gouspillou G et al. Anthracycline-containing chemotherapy causes long-term impairment of mitochondrial respiration and increased reactive oxygen species release in skeletal muscle. Sci Rep5:8717 (2015).
Read more (PubMed: 25732599) »
Davoudi M et al. Complex I function and supercomplex formation are preserved in liver mitochondria despite progressive complex III deficiency. PLoS One9:e86767 (2014).
Read more (PubMed: 24466228) »