概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SDF4
  • 特异性
    ab113413 is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Goat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    EYKVKFLASKGHSEK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 161-175 of Human SDF4 (NP_057631.1; NP_057260.2).

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab11343 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

靶标

Anti-SDF4 antibody 图像

  • ab113413 (5µg/ml) staining of paraffin embedded Human Kidney following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows preferential staining in the brush borders of epithelial cells.

  • Anti-SDF4 antibody (ab113413) at 0.1 µg/ml + Jurkat cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa

Anti-SDF4 antibody (ab113413)参考文献

ab113413 has not yet been referenced specifically in any publications.

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