Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
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Immunogen affinity purified
was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.
Ubiquitous. Isoform 5 is expressed in pancreas.
Belongs to the CREC family.
Contains 6 EF-hand domains.
Golgi apparatus lumen and Cytoplasm. Cell projection > bleb. Isoform 5 colocalizes with STX3 and STXBP1 isoform 2 at the plasma membrane and cell surface blebs.
Information by UniProt
45 kDa calcium-binding protein antibody
Anti-SDF4 antibody (ab113413)参考文献
has not yet been referenced specifically in any publications.
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