生物素Anti-SDF1抗体(ab84277)
Key features and details
- Biotin Rabbit polyclonal to SDF1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-SDF1抗体
参阅全部 SDF1 一抗 -
描述
生物素兔多克隆抗体to SDF1 -
宿主
Rabbit -
偶联物
Biotin -
特异性
The immunogen is 100% identical across P48061-1 to P48061-6. -
经测试应用
适用于: Sandwich ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human SDF1 aa 22-89. Produced from sera of rabbits pre-immunized with highly pure recombinant Human SDF-1a.
Sequence:KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Database link: P48061 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯化说明
ab84277 was purified by affinity chromatography and then biotinylated.It is sterile filtered. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Recombinant Protein
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to SDF1 (ab9797).
To detect Human SDF-1 alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ab9797 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1 alpha. |
|
WB |
Use at an assay dependent concentration.
|
说明 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to SDF1 (ab9797). To detect Human SDF-1 alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ab9797 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1 alpha. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. -
组织特异性
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
发展阶段
Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney. -
翻译后修饰
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6387 Human
- Omim: 600835 Human
- SwissProt: P48061 Human
- Unigene: 522891 Human
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别名
- 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
- AI174028 antibody
- C-X-C motif chemokine 12 antibody
see all
数据表及文件
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Datasheet download
文献 (0)
ab84277 尚未被引用在任何文献中。