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Our Abpromise guarantee covers the use of ab9797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1 alpha (100 ng/ml), a concentration of 2-4 µg/ml of this antibody is required.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21858102|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab84277 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha.|
|WB||Use at an assay dependent concentration. To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. To detect hSDF-1a by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1a.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent dilution.|
ab9797 staining SDF1 alpha in human colorectal adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
Immunohistochemical analysis of goat ovary tissue section, labelling SDF1 with ab9797. Samples were fixed in paraformaldhehyde, heat mediated antigen retrieval was performed with Citrate buffer, blocking was with BSA at 50µg/mL for 1 hour at 25°C. ab9797 was diluted 1/1000 and incubation was for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG H+L conjugated to Biotin (ab97049) diluted 1/500.
Immunohistochemical analysis of human colon tumour frozen section, labelling SDF1 with ab9797. Section was fixed in formaldehyde, permeabilzation was with 1x PBS with 0.3% triton-x-100, and blocking was with 5% serum for 1 hour at 24°C. ab9797 was diluted 1/1000 and incubation with sample was for 3 hours at 24°C. The secondary used was a Goat anti-Rabbit Alexa Fluor®488-conjugated polyclonal, diluted 1/2000. Nuclei stained red with DRAQ5.
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