概述

  • 产品名称Anti-SDF1抗体
    参阅全部 SDF1 一抗
  • 描述
    兔多克隆抗体to SDF1
  • 特异性This antibody recognizes SDF1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human SDF1 aa 44-93. A region within synthetic peptide: VKHLKILNTP NCALQIVARL KNNNRQVCID PKLKWIQEYL EKALNKRFKM, corresponding to amino acids 44-93 of Human SDF1
    Sequence:

    VKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKRFKM


    Database link: P48061

  • 阳性对照
    • Fetal lung lysate

性能

应用

Our Abpromise guarantee covers the use of ab49124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • 组织特异性Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 发展阶段Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • 翻译后修饰Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
    • AI174028 antibody
    • C-X-C motif chemokine 12 antibody
    • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
    • Chemokine (C-X-C motif) ligand 12 antibody
    • Chemokine CXC motif ligand 12 antibody
    • cxcl12 antibody
    • hIRH antibody
    • hSDF-1 antibody
    • Intercrine reduced in hepatomas antibody
    • IRH antibody
    • OTTHUMP00000019491 antibody
    • PBSF antibody
    • Pre-B cell growth-stimulating factor antibody
    • SCYB12 antibody
    • SDF 1 antibody
    • SDF-1 antibody
    • SDF-1-alpha(3-67) antibody
    • SDF-1a antibody
    • SDF-1b antibody
    • SDF1_HUMAN antibody
    • SDF1A antibody
    • SDF1B antibody
    • Stromal cell-derived factor 1 antibody
    • Stromal cell-derived factor 1 delta antibody
    • Stromal cell-derived factor 1 gamma antibody
    • Stromal cell-derived factor 1a antibody
    • Stromal cell-derived factor-1 alpha antibody
    • Thymic lymphoma cell-stimulating factor antibody
    • Tlsf antibody
    • TLSF-a antibody
    • TLSF-b antibody
    • Tlsfa antibody
    • Tlsfb antibody
    • TPAR1 antibody
    see all

Anti-SDF1 antibody 图像

  • Anti-SDF1 antibody (ab49124) at 0.5 µg/ml + Fetal lung lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 10 kDa

Anti-SDF1 antibody (ab49124)参考文献

This product has been referenced in:
  • Hedges EA  et al. Modulation of selectin-mediated adhesion of flowing lymphoma and bone marrow cells by immobilized SDF-1. Int J Mol Sci 15:15061-72 (2014). Read more (PubMed: 25167133) »

See 1 Publication for this product

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Each antibody should be titrated to determine the optimal working dilutions. It is the customer's responsibility to calculate how much product is needed for each assay.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole Lung)
Loading amount 50 µg
Specification Whole Lung
Gel Running Conditions Reduced Denaturing (15)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Apr 23 2008

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