概述

  • 产品名称Anti-SDF1抗体
    参阅全部 SDF1 一抗
  • 描述
    兔多克隆抗体to SDF1
  • 特异性This antibody should detect both alpha and beta isoforms.
  • 经测试应用适用于: IHC-Fr, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse SDF1 aa 1-93. Immunogen: Recombinant mouse SDF-1 beta
    Database link: P40224

性能

应用

Our Abpromise guarantee covers the use of ab25118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/500. PubMed: 19628780
IP 1/300 - 1/500.
WB 1/1000. Predicted molecular weight: 11 kDa.

靶标

  • 功能Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • 组织特异性Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 发展阶段Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • 翻译后修饰Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
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    • SDF-1a antibody
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    • SDF1_HUMAN antibody
    • SDF1A antibody
    • SDF1B antibody
    • Stromal cell-derived factor 1 antibody
    • Stromal cell-derived factor 1 delta antibody
    • Stromal cell-derived factor 1 gamma antibody
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    • Thymic lymphoma cell-stimulating factor antibody
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    • TLSF-a antibody
    • TLSF-b antibody
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Anti-SDF1 antibody (ab25118)参考文献

This product has been referenced in:
  • Tomas-Sanchez C  et al. Prophylactic Chronic Zinc Administration Increases Neuroinflammation in a Hypoxia-Ischemia Model. J Immunol Res 2016:4039837 (2016). ELISA ; Rat . Read more (PubMed: 27635404) »
  • Toshner M  et al. Evidence of dysfunction of endothelial progenitors in pulmonary arterial hypertension. Am J Respir Crit Care Med 180:780-7 (2009). Read more (PubMed: 19628780) »

See all 2 Publications for this product

Product Wall

We added IHC-P as a tested application from the reference below. In this reference, the authors describe the following antigen retrieval method:



Slides were microwaved for 30 minutes in a Milestone microwave (Surgipath, Peterboroug...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"