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Our Abpromise guarantee covers the use of ab25117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20871610
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 25086214|
|IHC-P||Use at an assay dependent concentration. PubMed: 21224081|
|WB||1/1000. Predicted molecular weight: 11 kDa.|
|IP||1/300 - 1/500.|
ab25117 staining SDF1 alpha in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate pH 6.0. Samples were then blocked with 10% serum for 30 minutes at 25°C and incubated with ab25117 at a 1/100 dilution for 2 hours at 25°C. The secondary used was an undiluted Alexa Fluor® 488-conjugated goat polyclonal.