概述

  • 产品名称Anti-SDF1抗体
    参阅全部 SDF1 一抗
  • 描述
    兔多克隆抗体to SDF1
  • 特异性The antibody should cross-react to both P40224-1 and P40224-2.
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Mouse SDF1 aa 1-93. E. coli-expressed mouse SDF1.
    Database link: P40224

性能

应用

Our Abpromise guarantee covers the use of ab18919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.
IP Use a concentration of 4 - 8 µg/ml.
IHC-P 1/25 - 1/50.
IHC-Fr 1/25 - 1/50.

靶标

  • 功能Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • 组织特异性Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 发展阶段Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • 翻译后修饰Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
    • AI174028 antibody
    • C-X-C motif chemokine 12 antibody
    • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
    • Chemokine (C-X-C motif) ligand 12 antibody
    • Chemokine CXC motif ligand 12 antibody
    • cxcl12 antibody
    • hIRH antibody
    • hSDF-1 antibody
    • Intercrine reduced in hepatomas antibody
    • IRH antibody
    • OTTHUMP00000019491 antibody
    • PBSF antibody
    • Pre-B cell growth-stimulating factor antibody
    • SCYB12 antibody
    • SDF 1 antibody
    • SDF-1 antibody
    • SDF-1-alpha(3-67) antibody
    • SDF-1a antibody
    • SDF-1b antibody
    • SDF1_HUMAN antibody
    • SDF1A antibody
    • SDF1B antibody
    • Stromal cell-derived factor 1 antibody
    • Stromal cell-derived factor 1 delta antibody
    • Stromal cell-derived factor 1 gamma antibody
    • Stromal cell-derived factor 1a antibody
    • Stromal cell-derived factor-1 alpha antibody
    • Thymic lymphoma cell-stimulating factor antibody
    • Tlsf antibody
    • TLSF-a antibody
    • TLSF-b antibody
    • Tlsfa antibody
    • Tlsfb antibody
    • TPAR1 antibody
    see all

Anti-SDF1 antibody 图像

  • ab18919 (2µg/ml) staining SDF1 in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secretory compartments within the smooth muscle fibres.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-SDF1 antibody (ab18919)参考文献

This product has been referenced in:
  • Gong HY  et al. Radiation-induced pulmonary injury accelerated pulmonary metastasis in a mouse model of breast cancer. Oncol Lett 10:3613-3618 (2015). IHC-P ; Mouse . Read more (PubMed: 26788178) »
  • Dominici M  et al. Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation. Blood 114:2333-43 (2009). IHC-P ; Mouse . Read more (PubMed: 19433859) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Hela, Hep G2)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Hela, Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jan 05 2015

Thank you for your enquiry. For paraffin-embedded tissue, heat in citrate pH 6.0 buffer for 5 - 10 minutes, heated to boiling either in a microwave or in a steamer. Please do not hesitate to contact us if you have any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"