Anti-SCYL1抗体(ab95074)
Key features and details
- Rabbit polyclonal to SCYL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SCYL1抗体
参阅全部 SCYL1 一抗 -
描述
兔多克隆抗体to SCYL1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region between residues 758 and 808 of Human SCYL1 (NP_065731.3).
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阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab95074 was affinity purified using an epitope specific to SCYL1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 90 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the protein kinase superfamily.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain. -
结构域
The protein kinase domain is predicted to be catalytically inactive. -
细胞定位
Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle. - Information by UniProt
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数据库链接
- Entrez Gene: 57410 Human
- Entrez Gene: 78891 Mouse
- Omim: 607982 Human
- SwissProt: Q96KG9 Human
- SwissProt: Q9EQC5 Mouse
- Unigene: 238839 Human
- Unigene: 276063 Mouse
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别名
- Coated vesicle associated kinase of 90 kDa antibody
- Coated vesicle-associated kinase of 90 kDa antibody
- CVAK90 antibody
see all
图片
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All lanes : Anti-SCYL1 antibody (ab95074) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 90 kDa
Exposure time: 10 seconds -
Detection of SCYL1 by Western Blot of Immunprecipitate.
ab95074 at 1µg/ml staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95074 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab95074 被引用在 1 文献中.
- Amano G et al. SCYL1 arginine methylation by PRMT1 is essential for neurite outgrowth via Golgi morphogenesis. Mol Biol Cell 31:1963-1973 (2020). PubMed: 32583741