概述

  • 产品名称
  • 描述
    兔多克隆抗体to SCXA
  • 经测试应用
    适用于: ICC/IF, WB, ELISA, IHC-M, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Horse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to N-terminal residues of human SCXA

性能

应用

Our Abpromise guarantee covers the use of ab58655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.Can be blocked with SCXA peptide (ab214116). IP enrichment could be done before running a reducing gel. The positive control is purified protein or overpressed cell lysate.
ELISA Use at an assay dependent concentration.
IHC-M Use at an assay dependent concentration. PubMed: 21412429
ICC Use at an assay dependent concentration. PubMed: 21412429

靶标

Anti-SCXA antibody (ab58655)参考文献

This product has been referenced in:
  • Spang C  et al. Glutamate signaling through the NMDA receptor reduces the expression of scleraxis in plantaris tendon derived cells. BMC Musculoskelet Disord 18:218 (2017). ICC/IF ; Human . Read more (PubMed: 28545490) »
  • Spang C  et al. The tenocyte phenotype of human primary tendon cells in vitro is reduced by glucocorticoids. BMC Musculoskelet Disord 17:467 (2016). ICC/IF ; Human . Read more (PubMed: 27832770) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (Heart)
Gel Running Conditions
Reduced Non-Denaturing (Native) (15)
Loading amount
50 µg
Treatment
TAC
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory Stem Cells of Rat (GFP))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rat (GFP)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

提交于 Jul 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

提交于 Jul 15 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization
Yes - 0,1 % Triton X100
Specification
Olfactory stem cells (tenocytes differentiation)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

提交于 Aug 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells)
Permeabilization
Yes - 0.1% Triton 100X
Specification
Olfactory stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

提交于 Jun 19 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Sample
Mouse Tissue sections (E11.5 embryos)
Specification
E11.5 embryos
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Tenocyte Differentiation)
Specification
Tenocyte Differentiation
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Tween 20
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 06 2013

Thank you for the blot image. I think the band below the 25 kDa marker is likely to be SCXA. The peptide blocking experiment might confirm this but the peptide might also block binding of the antibody to the other proteins at the higher molecular weigh...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Tendon)
Specification
Tendon
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton-X- in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Carsten Schnatwinkel

Verified customer

提交于 Jan 11 2013

IHC-M stands for Immunohistochemistry (Methylmethacrylate sections) and was sued in the publication with the following http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21412429&dopt=Abstract reference. Unfortun...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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