antigen, corresponding to amino acids 34-98 of Human SCRG1.
- Human cerebral cortex tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Expressed abundantly in the central nervous system of adult, but not at all in fetal brain. High levels of SCRG1 transcripts are also observed in testis and aorta.
Belongs to the SCRG1 family.
Information by UniProt
- AW124307 antibody
- Scrapie-responsive gene 1 protein antibody
- Scrapie-responsive protein 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SCRG1 antibody (ab121384)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab121384 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRG1 antibody (ab121384)
ab121384 at 1/100 dilution staining SCRG1 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry, showing strong cytoplasmic positivity in neuronal cells.
has not yet been referenced specifically in any publications.
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