Anti-Scramblase 1抗体[1E9] (ab24923)


  • 产品名称Anti-Scramblase 1抗体[1E9]
    参阅全部 Scramblase 1 一抗
  • 描述
    小鼠单克隆抗体[1E9] to Scramblase 1
  • 特异性ab24923 recognises Scramblase 1
  • 经测试应用适用于: Flow Cyt, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide from human Scramblase 1 protein.

  • 表位ab24923 reacts with an epitope located at the N terminus region of Scramblase 1
  • 阳性对照
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab24923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. For HRP/ECL detection. Note: Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Dilution optimised using Chromogenic detection.
ELISA Use a concentration of 0.1 µg/ml.


  • 功能May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
    May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.
  • 组织特异性Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
  • 序列相似性Belongs to the phospholipid scramblase family.
  • 翻译后修饰Known to be palmitoylated at one, yet undefined, site.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ca(2+) dependent phospholipid scramblase 1 antibody
    • Ca(2+)-dependent phospholipid scramblase 1 antibody
    • Erythrocyte phospholipid scramblase antibody
    • MM1 cell-derived transplantability-associated gene 1b, mouse, homolog of antibody
    • MmTRA1a antibody
    • MmTRA1b antibody
    • Nor1 antibody
    • Phospholipid scramblase 1 antibody
    • PL scramblase 1 antibody
    • PLS1_HUMAN antibody
    • PLSCR 1 antibody
    • Plscr1 antibody
    • Scramblase1 antibody
    • Tra1 antibody
    • Tra1a antibody
    • Tra1b antibody
    • Transplantability-associated protein 1 antibody
    • Tras1 antibody
    • Tras2 antibody
    see all

Anti-Scramblase 1 antibody [1E9] 图像

  • All lanes : Anti-Scramblase 1 antibody [1E9] (ab24923) at 0.5 µg/ml (Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane)

    Lane 1 : HeLa
    Lane 2 : HepG2
    Lane 3 : HEK-293
    Lane 4 : SY5Y
    Lane 5 : Jurkat
    Lane 6 : U937
    Lane 7 : K562
    Lane 8 : THP

    Lysates/proteins at 20000 cells per lane.

    A HRP-conjugated antibody
    Developed using the ECL technique

    Predicted band size : 35 kDa

    Exposure time : 30 seconds
  • Overlay histogram showing HeLa cells stained with ab24923 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24923, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Scramblase 1 antibody [1E9] (ab24923)参考文献

ab24923 has not yet been referenced specifically in any publications.

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We currently do not offer a blocking peptide for use with this antibody.