概述

  • 产品名称
  • 描述
    兔多克隆抗体to SCP3
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide (Human) (C terminal).

性能

应用

Our Abpromise guarantee covers the use of ab15093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/400.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/500.
IHC-FoFr 1/500. (see Abreview)

靶标

  • 功能
    Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
  • 组织特异性
    Testis-specific.
  • 疾病相关
    Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
  • 序列相似性
    Belongs to the XLR/SYCP3 family.
  • 细胞定位
    Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
  • Information by UniProt
  • 数据库链接
  • 别名
    • choline phosphotransferase 1 antibody
    • chpt1 antibody
    • COR 1 antibody
    • COR1 antibody
    • MGC71888 antibody
    • RNASCP3 antibody
    • SCP 3 antibody
    • SCP-3 antibody
    • SCP3 antibody
    • SPGF4 antibody
    • Sycp 3 antibody
    • Sycp3 antibody
    • SYCP3_HUMAN antibody
    • Synaptonemal complex protein 3 antibody
    see all

图片

  • SCP3 labeled in mouse pachytene preparation (red), using ab15093. CDK2 staining, near teleomeres, is also present (green).
  • ab15093 staining SCP3 in Mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150081 (1/500) was used as the secondary antibody.

    See Abreview

  • ab15093 staining SCP3 in Human TEFNA testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 5% FCS + 0.1% Triton X-100) for 1 hour at 25°C. Ab6721 (1/1000) was used as the secondary antibody.

    See Abreview

  • Ab15093 staining human normal testis tissue. Staining is localized to nuclear compartment.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab15093 staining SCP3 in Mouse testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at 1/200 dilution

  • ab15093 staining SCP3 (green) in Rat testis (spermatocytes) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS) for 2 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI (blue).

    See Abreview

  • ab15093 staining SCP3 in pig spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton x100 and blocking with 0.15% BSA was performed for 30 minutes at 250C. Samples were incubated with primary antibody (1/100: in PBT: PBS + 0.15% BSA+0.1% Tween 20) for 12 hours at 25°C. An Alexa Fluor®594-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.

    See Abreview

  • ab15093 staining SCP3 in Mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA and blocked with 5% BSA + 0.3% Triton X-100 for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An undiluted FITC-conjugated Donkey anti-Rabbit IgG (H+L) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Liu W  et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun 8:14182 (2017). WB ; Mouse . Read more (PubMed: 28128212) »
  • Marcet-Ortega M  et al. p53 and TAp63 participate in the recombination-dependent pachytene arrest in mouse spermatocytes. PLoS Genet 13:e1006845 (2017). ICC/IF ; Mouse . Read more (PubMed: 28617799) »

See all 70 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Spermatocytes)
Permeabilization
Yes - Tween-20
Specification
Spermatocytes
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Mar 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Other
Sample
Human Tissue sections (TEFNA human testis)
Specification
TEFNA human testis
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 26 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4% PFA
Username

Dr. Qing Wen

Verified customer

提交于 Apr 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4% PFA
Username

Dr. Qing Wen

Verified customer

提交于 May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 07 2013

Comme convenu j'ai mis en place l'envoi de 2 unités de ab15093, le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.<...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from this antibody.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human fetal ovary)
Specification
human fetal ovary
Fixative
neutral buffered formalin
Permeabilization
No
Blocking step
5% BSA / 20% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Testis (Spermatocytes))
Specification
Testis (Spermatocytes)
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 26 2010

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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