The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/750.
Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.
Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.
Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
Takata KI et al. Analysis of DNA polymerase ? function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet13:e1006818 (2017).
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