概述

  • 产品名称Anti-SCP1抗体
    参阅全部 SCP1 一抗
  • 描述
    兔多克隆抗体to SCP1
  • 经测试应用适用于: IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide (Mouse) (C terminal).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Tris citrate/phosphate. pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/750.
IHC-P 1/750.
ICC/IF 1/100 - 1/750.

靶标

  • 功能Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.
  • 组织特异性Testis.
  • 结构域Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.
  • 细胞定位Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 8 antibody
    • CT8 antibody
    • HOM TES 14 antibody
    • MGC104417 antibody
    • SCP 1 antibody
    • SCP-1 antibody
    • SCP1 antibody
    • SYCP 1 antibody
    • Sycp1 antibody
    • SYCP1_HUMAN antibody
    • Synaptonemal complex protein 1 antibody
    see all

Anti-SCP1 antibody 图像

  • SCP1 labeled in mouse pachytene preparation (red), using ab15090. CDK2 staining, near teleomeres, is also present (green).
  • Paraffin embeded IHC image of Murine SCP1 in mouse ovary using ab15090 at 1:750 dilution and secondary at 1:400.

Anti-SCP1 antibody (ab15090)参考文献

This product has been referenced in:
  • Chauhan S  et al. Cdk2 catalytic activity is essential for meiotic cell division in vivo. Biochem J 473:2783-98 (2016). Read more (PubMed: 27371320) »
  • Meng X  et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem 290:11853-64 (2015). IHC ; Mouse . Read more (PubMed: 25787082) »

See all 20 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (testis)
Permeabilization Yes - Triton
Specification testis
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse testes)
Specification mouse testes
Fixative 4% PFA
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 07 2013

Thank you for contacting Abcam.

Under our Abpromise we guarantee our products for 6 months from the date of delivery and so it is no longer covered under this guarantee. I will be more than happy to help you troubleshoot your protocol to see ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spermatocytes)
Specification Spermatocytes
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1%
Username

Abcam user community

Verified customer

提交于 May 02 2007

I can confirm that ab15090 is not likely to cross react with human as the immunogen shares only 83% homology with the human protein. Unfortunately ab15087 will work in human but does not appear to work in immunohistochemistry on paraffin-embedded sect...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"