概述

  • 产品名称Anti-SCNN1B抗体
    参阅全部 SCNN1B 一抗
  • 描述
    兔多克隆抗体to SCNN1B
  • 特异性Detects recombinant rat beta-epithelial sodium channel (SCNN1B).
  • 经测试应用适用于: IP, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rabbit
  • 免疫原

    Synthetic peptide corresponding to Human SCNN1B aa 619-640.
    Sequence:

    NYDSLRLQPLDVIESDSEGDAI


    (Peptide available as ab4926)

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

性能

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应用

Our Abpromise guarantee covers the use of ab2906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.

By immunoprecipitation this antibody specifically binds to unglycosylated, ~75 kDa recombinant rat SCNN1B protein.

IHC-P Use at an assay dependent concentration.

靶标

  • 功能Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • 疾病相关Pseudohypoaldosteronism 1, autosomal recessive
    Liddle syndrome
    Bronchiectasis with or without elevated sweat chloride 1
  • 序列相似性Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.
  • 翻译后修饰Phosphorylated on serine and threonine residues.
  • 细胞定位Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amiloride sensitive sodium channel subunit beta antibody
    • Amiloride-sensitive sodium channel subunit beta antibody
    • Beta NaCH antibody
    • Beta-ENaC antibody
    • Beta-NaCH antibody
    • ENaC beta antibody
    • ENaCB antibody
    • Epithelial Na(+) channel subunit beta antibody
    • Epithelial Na+ channel beta subunit antibody
    • Epithelial Na+ channel subunit beta antibody
    • Epithelial sodium channel beta 2 subunit antibody
    • Epithelial sodium channel beta 3 subunit antibody
    • Nonvoltage gated sodium channel 1 beta subunit antibody
    • Nonvoltage gated sodium channel 1 subunit beta antibody
    • Nonvoltage-gated sodium channel 1 subunit beta antibody
    • SCNEB antibody
    • SCNN 1B antibody
    • SCNN1B antibody
    • SCNNB_HUMAN antibody
    • Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody
    • Sodium channel nonvoltage gated 1 beta antibody
    see all

Anti-SCNN1B antibody 图像

  • IHC image of SCNN1B staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2906, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SCNN1B antibody (ab2906)参考文献

This product has been referenced in:
  • Zhou Y  et al. Induction of renal 20-hydroxyeicosatetraenoic acid by clofibrate attenuates high-fat diet-induced hypertension in rats. J Pharmacol Exp Ther 317:11-8 (2006). Read more (PubMed: 16339392) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 40 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"