1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Voltage dependent sodium channels mediate the sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, they form a sodium selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. These ion channel proteins exist as heteromultimeric complexes of a large alpha subunit and 1 or 2 smaller beta subunits. SCN7A (Sodium channel protein type 7 subunit alpha) is an alpha subunit and is expressed in heart and uterus.
Putative voltage gated sodium channel subunit alpha Nax antibody
SCN 6A antibody
SCN 7A antibody
Sodium channel protein cardiac and skeletal muscle subunit alpha antibody
Sodium channel protein type 7 subunit alpha antibody
Sodium channel protein type VII subunit alpha antibody
Sodium channel voltage gated type VI alpha polypeptide antibody
Sodium channel voltage gated type VII alpha antibody
Sodium channel voltage gated type VII alpha polypeptide antibody
Voltage dependent sodium channel alpha subunit antibody
Immunocytochemistry/ Immunofluorescence - Anti-SCN7A antibody (ab66499)Image from Pinto FM et al., Reprod Biol Endocrinol. 2009 Jul 16;7:71. Fig 2.; doi:10.1186/1477-7827-7-71; 16 July, 2009
ab66499 staining SCN7A in Human sperm cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol and blocked with 2% BSA in PBS for 30 min. Samples were incubated with primary antibody (1/100 in 2% BSA in PBS) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.
Lara LS et al. The sodium-activated sodium channel is expressed in the rat kidney thick ascending limb and collecting duct cells and is upregulated during high salt intake. Am J Physiol Renal Physiol303:F105-9 (2012).
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Ke CB et al. Enhanced SCN7A/Nax expression contributes to bone cancer pain by increasing excitability of neurons in dorsal root ganglion. Neuroscience227C:80-89 (2012).
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