快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SCN4B

    该产品是 快线抗体,通过亲和纯化得到。对抗原肽有高灵敏度及特异性(ELISA测试)。 Read the terms of use »

  • 宿主
    Rabbit
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SCN4B.

    (Peptide available as ab95413.)

  • 阳性对照
    • Brain (Human) Tissue Lysate; Brain (Rat) Tissue Lysate ; U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate ; Spinal Cord (Human) Tissue Lysate; Spinal Cord (Rat) Tissue Lysate ; Skeletal Muscle (Human) Tissue Lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    The SCN4B protein modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation By similarity. The protein has been found to be expressed at a high level in dorsal root ganglia, at a lower level in brain, spinal cord, skeletal muscle and heart. Defects in SCN4B are the cause of long QT syndrome type 10 (LQT10) [MIM:611819]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
  • 细胞定位
    Cell Membrane
  • 数据库链接
  • 别名
    • SCN4B_HUMAN antibody
    • Sodium channel subunit beta-4 antibody

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-SCN4B antibody (ab80539) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 5 : Spinal Cord (Rat) Tissue Lysate
    Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. SCN4B contains at least three potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted. The primary sequence contains a 30-residue signal sequence at the N-terminus. The band around 20-kDa in human skeletal muscle may be the unglycosylated form of the target protein.
  • ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Barbosa C  et al. Navß4 regulates fast resurgent sodium currents and excitability in sensory neurons. Mol Pain 11:60 (2015). Read more (PubMed: 26408173) »
  • Tan ZY  et al. Tetrodotoxin-resistant sodium channels in sensory neurons generate slow resurgent currents that are enhanced by inflammatory mediators. J Neurosci 34:7190-7 (2014). Read more (PubMed: 24849353) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 10 2015

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (9% acrylamide)
Sample
Rat Tissue lysate - other (muscle)
Specification
muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Mr. Baptiste Jude

Verified customer

提交于 Feb 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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