概述

  • 产品名称Anti-SCN3A抗体
    参阅全部 SCN3A 一抗
  • 描述
    兔多克隆抗体to SCN3A
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human SCN3A aa 80-130.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 226 kDa.

靶标

  • 功能Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • 序列相似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.3/SCN3A subfamily.
    Contains 1 IQ domain.
  • 结构域The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻译后修饰May be ubiquitinated by NEDD4L; which would promote its endocytosis.
    Phosphorylation at Ser-1501 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • brain III voltage-gated sodium channel antibody
    • NAC3 antibody
    • Nav1.3 antibody
    • SCN3A antibody
    • SCN3A_HUMAN antibody
    • Sodium channel protein brain III subunit alpha antibody
    • Sodium channel protein type 3 subunit alpha antibody
    • Sodium channel protein type III subunit alpha antibody
    • Sodium channel protein, brain III subunit alpha antibody
    • sodium channel, brain type III, alpha subunit antibody
    • sodium channel, neuronal type III, alpha subunit antibody
    • sodium channel, voltage-gated, type III, alpha polypeptide antibody
    • sodium channel, voltage-gated, type III, alpha subunit antibody
    • Voltage gated sodium channel subtype III antibody
    • Voltage gated sodium channel subunit alpha Nav1.3 antibody
    • Voltage-gated sodium channel subtype III antibody
    • Voltage-gated sodium channel subunit alpha Nav1.3 antibody
    see all

Anti-SCN3A antibody 图像

  • ICC/IF image of ab65164 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65164, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SCN3A antibody (ab65164)参考文献

ab65164 has not yet been referenced specifically in any publications.

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