Anti-SCN2A抗体(ab65163)

概述

  • 产品名称Anti-SCN2A抗体
    参阅全部 SCN2A 一抗
  • 描述
    兔多克隆抗体to SCN2A
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the N terminal of human SCN2A.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 228 kDa.

靶标

  • 功能Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • 疾病相关Defects in SCN2A are a cause of generalized epilepsy with febrile seizures plus (GEFS+) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
    Defects in SCN2A are the cause of benign familial infantile convulsions type 3 (BFIC3) [MIM:607745]. BFIC3 is an autosomal dominant disorder in which afebrile seizures occur in clusters during the first year of life, without neurologic sequelae.
    Defects in SCN2A are the cause of epileptic encephalopathy early infantile type 11 (EIEE11) [MIM:613721]. EIEE11 is an autosomal dominant seizure disorder characterized by infantile onset of refractory seizures with resultant delayed neurologic development and persistent neurologic abnormalities.
  • 序列相似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.2/SCN2A subfamily.
    Contains 1 IQ domain.
  • 结构域The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻译后修饰May be ubiquitinated by NEDD4L; which would promote its endocytosis.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HBSC II antibody
    • NAC2 antibody
    • Scn2a antibody
    • SCN2A_HUMAN antibody
    • SCN2A1 antibody
    • SCN2A2 antibody
    • Sodium channel protein brain II subunit alpha antibody
    • Sodium channel protein type 2 subunit alpha antibody
    • Sodium channel protein type II subunit alpha antibody
    • Sodium channel protein, brain II subunit alpha antibody
    • Voltage gated sodium channel subunit alpha Nav1.2 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.2 antibody
    see all

Anti-SCN2A antibody 图像

  • ICC/IF image of ab65163 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65163, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SCN2A antibody (ab65163)参考文献

ab65163 has not yet been referenced specifically in any publications.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Hippocampal synaptosome preparation)
Loading amount 2 µg
Specification Hippocampal synaptosome preparation
Gel Running Conditions Reduced Denaturing (Bis Tris 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 08 2010

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