概述

  • 产品名称
  • 描述
    兔多克隆抗体to SCN11A
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from N terminal squence of human SCN11A.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-P
IHC-Fr
WB
  • 应用说明
    ICC/IF: Use at an assay dependent dilution (PMID 19607678).
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 205 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Also involved, with the contribution of the receptor tyrosine kinase NTRK2, in rapid BDNF-evoked neuronal depolarization.
    • 组织特异性
      Expressed in the dorsal root ganglia and trigeminal ganglia, olfactory bulb, hippocampus, cerebellar cortex, spinal cord, spleen, small intestine and placenta.
    • 疾病相关
      Neuropathy, hereditary sensory and autonomic, 7
      Episodic pain syndrome, familial, 3
    • 序列相似性
      Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.9/SCN11A subfamily.
    • 结构域
      The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
    • 翻译后修饰
      Phosphorylation at Ser-1341 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hNaN antibody
      • NaN antibody
      • NAV1.9 antibody
      • Peripheral nerve sodium channel 5 antibody
      • PN 5 antibody
      • PN5 antibody
      • SCN 11A antibody
      • SCN 12A antibody
      • Scn11a antibody
      • SCN12A antibody
      • SCNBA_HUMAN antibody
      • Sensory neuron sodium channel 2 antibody
      • SNS 2 antibody
      • SNS2 antibody
      • Sodium channel protein type 11 subunit alpha antibody
      • Sodium channel protein type XI subunit alpha antibody
      • Sodium channel voltage gated type XI alpha antibody
      • Sodium channel voltage gated type XI alpha polypeptide antibody
      • Sodium channel voltage gated type XI alpha subunit antibody
      • Sodium channel voltage gated type XII alpha polypeptide antibody
      • Voltage gated sodium channel Nav1.9 antibody
      • Voltage gated sodium channel subunit alpha Nav1.9 antibody
      • Voltage-gated sodium channel subunit alpha Nav1.9 antibody
      see all

    图片

    • ab65160 staining SCN11A in human sperm cells by Immunocytochemistry/ Immunofluorescence.
      Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab65160 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.

    文献

    This product has been referenced in:
    • Pinto FM  et al. Molecular and functional characterization of voltage-gated sodium channels in human sperm. Reprod Biol Endocrinol 7:71 (2009). ICC/IF ; Human . Read more (PubMed: 19607678) »

    See 1 Publication for this product

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