Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
Belongs to the SCM family. Contains 2 MBT repeats. Contains 1 SAM (sterile alpha motif) domain.
Sex comb on midleg, Drosophila, homolog-like 2 antibody
Sex comb on midleg-like protein 2 antibody
Western blot - SCML2 antibody (ab115160)
All lanes : Anti-SCML2 antibody (ab115160) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : 293T cell lysate at 50 µg Lane 4 : Jurkat cell lysate at 50 µg
Predicted band size : 77 kDa
Exposure time : 3 minutes
Immunoprecipitation - SCML2 antibody (ab115160)
Immunoprecipitation of SCML2 (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-SCML2 antibody that recognizes an upstream epitope (ab115157); Lane 2-Immunoprecipitation with anti-SCML2 antibody (ab115160); Lane 3- Immunoprecipitation with IgG control. Anti-SCML2 antibody (ab115160) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.