The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
组织特异性Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
序列相似性Belongs to the SCM family. Contains 2 MBT repeats. Contains 1 SAM (sterile alpha motif) domain.
Immunoprecipitation of SCML2 (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-SCML2 antibody (ab115157); Lane 2-Immunoprecipitation with an anti-SCML2 antibody that recognizes an downstream epitope (ab115160) ; Lane 3- Immunoprecipitation with IgG control.
Anti-SCML2 antibody from lane 2 (ab1151600) was used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 3o sec.
Anti-SCML2 antibody (ab115157)参考文献
has not yet been referenced specifically in any publications.