The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
Belongs to the SCM family. Contains 2 MBT repeats. Contains 1 SAM (sterile alpha motif) domain.
Immunoprecipitation of SCML2 (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-SCML2 antibody (ab115157); Lane 2-Immunoprecipitation with an anti-SCML2 antibody that recognizes an downstream epitope (ab115160) ; Lane 3- Immunoprecipitation with IgG control.
Anti-SCML2 antibody from lane 2 (ab1151600) was used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 3o sec.