概述

  • 产品名称Anti-SCML1抗体
    参阅全部 SCML1 一抗
  • 描述
    兔多克隆抗体to SCML1
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (ESYSPTLPVS RRENNSPSNL PRPSFCMEEY QRAELEEDPI LSRTPSPVHP) of Human SCML1 (NP_006737).

  • 阳性对照
    • HeLa cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

靶标

  • 功能Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. May be involved in spermatogenesis during sexual maturation.
  • 组织特异性Ubiquitous. Expressed in fetal and adult tissues.
  • 序列相似性Belongs to the SCM family.
    Contains 1 SAM (sterile alpha motif) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gm1792 antibody
    • Gm47 antibody
    • OTTHUMP00000022996 antibody
    • OTTHUMP00000025692 antibody
    • RP3-389A20.1 antibody
    • SCML1 antibody
    • SCML1_HUMAN antibody
    • Sex comb on midleg (Drosophila) like 1 antibody
    • Sex comb on midleg like 1 (Drosophila) antibody
    • Sex comb on midleg like 1 antibody
    • Sex comb on midleg like protein 1 antibody
    • Sex comb on midleg-like protein 1 antibody
    • Vsex comb on midleg like 1 antibody
    see all

Anti-SCML1 antibody 图像

  • Anti-SCML1 antibody (ab83665) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SCML1 with ab83665 at 5.0µg/ml.

Anti-SCML1 antibody (ab83665)参考文献

ab83665 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83665.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"