概述

  • 产品名称Anti-SCMH1抗体
    参阅全部 SCMH1 一抗
  • 描述
    小鼠单克隆抗体to SCMH1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Cynomolgus Monkey
  • 免疫原

    Recombinant fragment: EKLCHNLRSD NLFGNQPFTQ THLSLTAIEY SHSHDRYLPG ETFVLGNSLA RSLEPHSDSM DSASNPTNLV STSQRHRPLL SSCGLPPSTA SAVRRLCSR, corresponding to amino acids 404-502 of Human SCMH1 (NP_036368) with proprietory tag.

  • 阳性对照
    • HepG2 cell lysate. Recombinant tagged human SCMH1 fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab77648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged SCMH1 is approximately 30ng/ml when used as a capture antibody.

靶标

  • 功能Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 组织特异性Strongly expressed in heart, muscle and pancreas. Weakly expressed in brain, placenta, lung, liver and kidney.
  • 序列相似性Belongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Polycomb protein SCMH1 antibody
    • SCMH 1 antibody
    • SCMH1 antibody
    • SCMH1_HUMAN antibody
    • Scml 3 antibody
    • Scml3 antibody
    • Sex comb on midleg 1 antibody
    • Sex comb on midleg homolog 1 antibody
    see all

Anti-SCMH1 antibody 图像

  • Anti-SCMH1 antibody (ab77648) at 1 µg/ml + Recombinant tagged human SCMH1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-SCMH1 antibody (ab77648) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-SCMH1 antibody (ab77648)参考文献

ab77648 has not yet been referenced specifically in any publications.

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