概述

  • 产品名称Anti-Sclerostin抗体
    参阅全部 Sclerostin 一抗
  • 描述
    兔多克隆抗体to Sclerostin
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human
  • 免疫原

    Synthetic peptide corresponding to Human Sclerostin aa 12-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 50964

  • 阳性对照
    • WB: HeLa cell lysate. Mouse liver, mouse lung, rat kidney and human kidney tissue lysates. IHC-P: Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/10 - 1/50.

靶标

  • 功能Negative regulator of bone growth.
  • 组织特异性Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days.
  • 疾病相关Defects in SOST are the cause of sclerosteosis (SOST) [MIM:269500]; also known as cortical hyperostosis with syndactyly. SOST is an autosomal recessive sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients.
    Note=A 52 kb deletion downstream of SOST results in SOST transcription suppression and is a cause of van Buchem disease (VBCH) [MIM:239100]; also known as hyperostosis corticalis generalisata. VBCH is an autosomal recessive sclerosing bone dysplasia characterized by endosteal hyperostosis of the mandible, skull, ribs, clavicles, and diaphyses of the long bones. Affected patients present a symmetrically increased thickness of bones, most frequently found as an enlarged jawbone, but also an enlargement of the skull, ribs, diaphysis of long bones, as well as tubular bones of hands and feet. The clinical consequence of increased thickness of the skull include facial nerve palsy causing hearing loss, visual problems, neurological pain, and, very rarely, blindness as a consequence of optic atrophy. Serum alkaline phosphatase levels are elevated.
  • 序列相似性Belongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BEER antibody
    • CDD antibody
    • Cortical hyperostosis with syndactyly antibody
    • Sclerosteosis antibody
    • Sclerostin antibody
    • Sost antibody
    • SOST_HUMAN antibody
    • SOST1 antibody
    • UNQ2976/PRO7455/PRO7476 antibody
    • VBCH antibody
    see all

Anti-Sclerostin antibody 图像

  • All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

    Predicted band size : 24 kDa
  • Anti-Sclerostin antibody (ab63097) at 1/1000 dilution + HeLa cell lysate at 35 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 24 kDa
  • All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 24 kDa
  • All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 24 kDa
  • Anti-Sclerostin antibody (ab63097) at 1/500 dilution + Rat bone (tibia) whole tissue lysate at 36 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 55 kDa (possible non-specific binding),60 kDa (possible non-specific binding).

    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Helder Fonseca

    See Abreview

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling sclerostin with ab63097 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Sclerostin with ab63097. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-Sclerostin antibody (ab63097)参考文献

This product has been referenced in:
  • Cohen-Kfir E  et al. Sirt1 is a regulator of bone mass and a repressor of Sost encoding for sclerostin, a bone formation inhibitor. Endocrinology 152:4514-24 (2011). Mouse . Read more (PubMed: 21952235) »
  • Mardaryev AN  et al. Micro-RNA-31 controls hair cycle-associated changes in gene expression programs of the skin and hair follicle. FASEB J 24:3869-81 (2010). WB ; Mouse . Read more (PubMed: 20522784) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization Yes - 0.025% Triton x-100
Specification Bone tissue (Femur)
Blocking step Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 11 2015

Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Rat Tissue lysate - whole (Bone - Tibia)
Specification Bone - Tibia
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Helder Fonseca

Verified customer

提交于 Sep 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW1353 human chondrosacroma cell line)
Loading amount 40 µg
Specification SW1353 human chondrosacroma cell line
Treatment Transfected with full length human SOST
Gel Running Conditions Reduced Denaturing (10% Bis-Tris Gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2013

Thank you for contacting Abcam.

The western blot image for ab63097 was provided by the supplying laboratory of this product. The antibody was not one that was produced by Abcam, however we are confident about the data that is shown on our web...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Bone and cartilage)
Specification Bone and cartilage
Fixative 10% neutral-buffered formalin
Antigen retrieval step None
Permeabilization No
Blocking step Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"