概述

  • 产品名称
    Anti-SCFD1抗体
    参阅全部 SCFD1 一抗
  • 描述
    兔多克隆抗体to SCFD1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 300 - 350 (VENSPAGARP KRKNKKSYDL TPVDKFWQKH KGSPFPEVAE SVQQELESYR A) of Human SCFD1 (NP_057190.2)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Whole cell lysate from HeLa cells, 293T cells or NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab86594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.
  • 序列相似性
    Belongs to the STXBP/unc-18/SEC1 family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • C14orf163 antibody
    • Chromosome 14 open reading frame 163 antibody
    • RA410 antibody
    • Scfd1 antibody
    • SCFD1_HUMAN antibody
    • Sec1 family domain containing 1 antibody
    • Sec1 family domain-containing protein 1 antibody
    • SLY1 antibody
    • SLY1 homolog antibody
    • Sly1p antibody
    • STXBP1L2 antibody
    • Syntaxin binding protein 1 like 2 antibody
    • Syntaxin-binding protein 1-like 2 antibody
    • Vesicle transport related protein antibody
    see all

图片

  • Western blot - SCFD1 antibody (ab86594)
    Western blot - SCFD1 antibody (ab86594)
    All lanes : Anti-SCFD1 antibody (ab86594) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Predicted band size: 72 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - SCFD1 antibody (ab86594)
    Immunoprecipitation - SCFD1 antibody (ab86594)
    1mg whole cell lysate from Hela cells was immunoprecipited using 3ug ab86594. 20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.

文献

ab86594 has not yet been referenced specifically in any publications.

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