重组
RabMAb

Anti-SCF抗体[EP665Y] (ab52603)

概述

  • 产品名称
    Anti-SCF抗体[EP665Y]
    参阅全部 SCF 一抗
  • 描述
    兔单克隆抗体[EP665Y] to SCF
  • 经测试应用
    适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human SCF.

  • 阳性对照
    • Human kidney carcinoma tissue.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/20 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 疾病相关
    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 序列相似性
    Belongs to the SCF family.
  • 发展阶段
    Acts in the early stages of hematopoiesis.
  • 翻译后修饰
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 细胞定位
    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C kit ligand antibody
    • C-kit ligand antibody
    • Ckit ligand antibody
    • DCUA antibody
    • DFNA69 antibody
    • DKFZp686F2250 antibody
    • familial progressive hyperpigmentation 2 antibody
    • FPH2 antibody
    • FPHH antibody
    • KIT ligand antibody
    • Kitl antibody
    • KITLG antibody
    • KL 1 antibody
    • KL1 antibody
    • Mast cell growth factor antibody
    • MGF antibody
    • MGF stem cell factor antibody
    • SCF antibody
    • SCF_HUMAN antibody
    • SF antibody
    • SHEP7 antibody
    • sKITLG antibody
    • Soluble KIT ligand antibody
    • Steel factor antibody
    • steel, mouse, homolog of antibody
    • Stem cell factor antibody
    • Stem cell factor precursor antibody
    see all

图片

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

  • Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)

    Secondary
    HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 31 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250.
  • Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

This product has been referenced in:
  • Yong KS  et al. Human CD34(lo)CD133(lo) fetal liver cells support the expansion of human CD34(hi)CD133(hi) hematopoietic stem cells. Cell Mol Immunol 13:605-14 (2016). ICC/IF . Read more (PubMed: 27593483) »
  • Huang X  et al. Three-dimensional co-culture of mesenchymal stromal cells and differentiated osteoblasts on human bio-derived bone scaffolds supports active multi-lineage hematopoiesis in vitro: Functional implication of the biomimetic HSC niche. Int J Mol Med 38:1141-51 (2016). WB . Read more (PubMed: 27571775) »

See all 8 Publications for this product

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine cell lines, tissues and recombinant canine)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
50 µg
Specification
Canine cell lines, tissues and recombinant canine
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Permeabilization
Yes - 1%tween
Specification
NIH3T3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 25 2015

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Denaturing (14%)
Sample
Human Cell lysate - whole cell (CLL cells)
Specification
CLL cells
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Mr. George Gavriilidis

Verified customer

提交于 Feb 26 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Serum (serum)
Specification
serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Oct 31 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5%
Sample
Human Cell (H9 embryonic stem cells)
Specification
H9 embryonic stem cells
Permeabilization
Yes - 0.01% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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