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Our Abpromise guarantee covers the use of ab9716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use at an assay dependent dilution. Allows the detection of 0.2 - 0.4 ng/well of recombinant hSCF (using 100µl/well antibody solution).|
|Neutralising||Use a concentration of 0.008 - 0.012 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hSCF (10.0 ng/ml).|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
ab9716 staining SCF in human pancreatic adenocarcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent proteinase K mediated antigen retrieval at room temperature. The primary antibody was used at 0.5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"