概述

  • 产品名称
  • 描述
    兔多克隆抗体to SCF
  • 特异性
    Recent retest of the latest lot of this antibody have shown that this product cannot be longer guaranteed in WB using cell lines and tissue lysates expressing the endogenous protein only. Instead batches have passed in WB using recombinant SCF lysate. All batches are still tested successfully in both ICC and IHC (endogenous protein). (Sept 2017)
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human, African green monkey
    预测可用于: Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCF.

    (Peptide available as ab73180.)

  • 阳性对照
    • This antibody gave a positive signal in recombinant SCF protein. ICC/IF: HepG2 cells. IHC: Human normal colon

性能

应用

Our Abpromise guarantee covers the use of ab64677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 31 kDa).

靶标

  • 功能
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 疾病相关
    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 序列相似性
    Belongs to the SCF family.
  • 发展阶段
    Acts in the early stages of hematopoiesis.
  • 翻译后修饰
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 细胞定位
    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C kit ligand antibody
    • C-kit ligand antibody
    • Ckit ligand antibody
    • DCUA antibody
    • DFNA69 antibody
    • DKFZp686F2250 antibody
    • familial progressive hyperpigmentation 2 antibody
    • FPH2 antibody
    • FPHH antibody
    • KIT ligand antibody
    • Kitl antibody
    • KITLG antibody
    • KL 1 antibody
    • KL1 antibody
    • Mast cell growth factor antibody
    • MGF antibody
    • MGF stem cell factor antibody
    • SCF antibody
    • SCF_HUMAN antibody
    • SF antibody
    • SHEP7 antibody
    • sKITLG antibody
    • Soluble KIT ligand antibody
    • Steel factor antibody
    • steel, mouse, homolog of antibody
    • Stem cell factor antibody
    • Stem cell factor precursor antibody
    see all

Anti-SCF antibody 图像

  • ab64677 stained in HepG2 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab64677 at 5 µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature

  • IHC image of SCF staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64677, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Anti-SCF antibody (ab64677) at 1 µg/ml + SCF Recombinant Protein at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

Anti-SCF antibody (ab64677)参考文献

This product has been referenced in:
  • Souilhol C  et al. Inductive interactions mediated by interplay of asymmetric signalling underlie development of adult haematopoietic stem cells. Nat Commun 7:10784 (2016). IF ; Mouse . Read more (PubMed: 26952187) »
  • Imsland F  et al. Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses. Nat Genet 48:152-8 (2016). Read more (PubMed: 26691985) »

See all 8 Publications for this product

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DISCOUNT CODE: ***
Expiration date: June 11, 2012

This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forab64677 with mousesamplesand include this code in the “A...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (COS cell line)
Specification
COS cell line
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 02 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Ovary)
Specification
Mouse Ovary
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris Buffer pH 10.0
Permeabilization
Yes - Tween20
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 May 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293FT cell line)
Specification
293FT cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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