概述

  • 产品名称Anti-SCF抗体
    参阅全部 SCF 一抗
  • 描述
    山羊多克隆抗体to SCF
  • 经测试应用适用于: Neutralising, WB, Indirect ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Highly pure (>98%) recombinant Rat SCF.

  • 阳性对照
    • Recombinant Rat SCF

性能

应用

Our Abpromise guarantee covers the use of ab101072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use a concentration of 0.08 - 0.12 µg/ml. Required to yield one half maximal inhibition [ND50] of the biological activity of Rat SCF (15 ng/ml)
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat SCF is 1.5-3.0 ng/lane, under either reducing or non reducing conditions.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. Using 100 µl/well antibody solution, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SCF in conjunction with compatible secondary reagents.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Using 100 µl/well antibody solution, in conjunction with Biotinylated Anti-Rat SCF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SCF.

靶标

  • 功能Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 疾病相关Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 序列相似性Belongs to the SCF family.
  • 发展阶段Acts in the early stages of hematopoiesis.
  • 翻译后修饰A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 细胞定位Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C kit ligand antibody
    • C-kit ligand antibody
    • Ckit ligand antibody
    • DCUA antibody
    • DFNA69 antibody
    • DKFZp686F2250 antibody
    • familial progressive hyperpigmentation 2 antibody
    • FPH2 antibody
    • FPHH antibody
    • KIT ligand antibody
    • Kitl antibody
    • KITLG antibody
    • KL 1 antibody
    • KL1 antibody
    • Mast cell growth factor antibody
    • MGF antibody
    • MGF stem cell factor antibody
    • SCF antibody
    • SCF_HUMAN antibody
    • SF antibody
    • SHEP7 antibody
    • sKITLG antibody
    • Soluble KIT ligand antibody
    • Steel factor antibody
    • steel, mouse, homolog of antibody
    • Stem cell factor antibody
    • Stem cell factor precursor antibody
    see all

Anti-SCF antibody (ab101072)参考文献

ab101072 has not yet been referenced specifically in any publications.

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