概述

  • 产品名称Anti-SCE1抗体
    参阅全部 SCE1 一抗
  • 描述
    兔多克隆抗体to SCE1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Arabidopsis thaliana
  • 免疫原

    Full length protein produced in E.coli.

性能

应用

Our Abpromise guarantee covers the use of ab98965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 18 kDa.

靶标

  • 相关性SUMO-conjugating enzyme accepts the SUMO proteins from the E1 SUMO-activating heterodimer SAE1/SAE2 and catalyzes its covalent attachment to other proteins with the E3 SUMO ligases SIZ1 and MMS21. It associates with SIZ1 for sumoylation of the transcription factor GTE3.
  • 数据库链接
    • 别名
      • AHUS5 antibody
      • AT3G57870 antibody
      • ATSCE1 antibody
      • EMB1637 antibody
      • EMBRYO DEFECTIVE 1637 antibody
      • SCE1A antibody
      • SUMO CONJUGATING ENZYME 1A antibody
      • SUMO CONJUGATION ENZYME 1 antibody
      • SUMO ligase antibody
      see all

    Anti-SCE1 antibody 图像

    • All lanes : Anti-SCE1 antibody (ab98965) at 1/1000 dilution

      Lane 1 : Whole leaf tissue lysate prepared from wild type Arabidopsis thaliana Col-0
      Lane 2 : Whole leaf tissue lysate prepared from Arabidopsis thaliana overexpressing SCE1

      Secondary
      HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
      Additional bands at : 28 kDa,32 kDa,50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

      Image courtesy of Michael Skelly by Abreview.

      See Abreview

    Anti-SCE1 antibody (ab98965)参考文献

    ab98965 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Arabidopsis thaliana Tissue lysate - whole (Arabidopsis leaf tissue)
    Loading amount 200000 µg
    Specification Arabidopsis leaf tissue
    Gel Running Conditions Non-reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Mr. Michael Skelly

    Verified customer

    提交于 Aug 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"